Vivian Agincourt Spence Little was born to parents Edward Agincourt and Alice Little (Horley) on 5 April 1878. He received his education at Cleveland Street School and Sydney High School, before earning his Bachelor of Arts in 1903 and Masters of Arts in 1907 from Sydney University. He also earned a Bachelor of Letters from Oxford. In 1903, Reverend Little entered the Methodist Ministry..
In 1912, he joined the Royal Australian Navy and was appointed to HMAS Encounter and HMAS Cerberus. Reverend Little was the first non-Anglican Protestant chaplain to be appointed in the RAN. In July 1913, Little was appointed as chaplain for HMAS Sydney. He was serving on HMAS Sydney during the Battle of Rabaul and the battle with the SMS Emden on 9 November 1914. Reverend Little served on HMAS Sydney throughout the war and was discharged at his own request in June 1917.
In 1921, he married Ethel Maud Lock and they had two children, Beatrice Ethel Spence Little and Wardlow Agincourt Spence Little. In 1934, Reverend Vivian Little published the book The Christology of the apologists: doctrinal with an introduction by W. B. Selbie.
Vivian Agincourt Spence Little died on 25 March 1956 in Sydney, New South Wales.